Fluorescence energy transfer detection as a homogeneous DNA diagnostic method
نویسندگان
چکیده
منابع مشابه
Fluorescence energy transfer detection as a homogeneous DNA diagnostic method.
A homogeneous DNA diagnostic assay based on template-directed primer extension detected by fluorescence resonance energy transfer, named template-directed dye-terminator incorporation (TDI) assay, has been developed for mutation detection and high throughput genome analysis. Here, we report the successful application of the TDI assay to detect mutations in the cystic fibrosis transmembrane cond...
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A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence resonance energy transfer is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a 5'-fluorescein-labeled primer (designed to hybridize to the DNA template adjacent to the polymorphic site) in the presence of allelic dye-labeled dideox...
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Direct or indirect interactions between membrane proteins at the cell surface play a central role in numerous cell processes, including possible synergistic effects between different types of receptors. Here we describe a method and tools to analyze membrane protein-protein interaction at the surface of living cells. This technology is based on the use of specific antibodies directed against ea...
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The first step in transcriptional activation of protein-coding genes involves the assembly on the promoter of a large PIC (pre-initiation complex) comprising RNA polymerase II and a suite of general transcription factors. Transcription is greatly enhanced by the action of promoter-specific activator proteins (activators) that function, at least in part, by increasing PIC formation. Activator-me...
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DNA is a unique molecule that can be used to separate donor and acceptor for fluorescence energy transfer (ET)1 and longrange photoinduced electron-transfer studies.2 ET has been used extensively as a spectroscopic ruler for biological structures,3a-c and ET primers and terminators are markedly superior to single dye-labeled reagents in DNA sequencing and analysis.4a-b Molecular beacons using b...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.20.10756